Journal: The Oncologist

Article Title: First-Line Osimertinib in Patients with EGFR -Mutant Advanced Non-Small Cell Lung Cancer: Outcome and Safety in the Real World: FLOWER Study

doi: 10.1002/onco.13951

Figure Lengend Snippet: Univariate and multivariate analysis for progression free survival, overall survival and time to discontinuation according to patients features (Log-rank test and Cox proportional hazard)

Article Snippet: For analyses on tissue sample, tumor DNA was extracted from formalin-fixed paraffin-embedded (FFPE) tumor slices through QIAamp DNA FFPE kit (Qiagen, Hilden, Germany); DNA sequencing was carried out with Sanger sequencing, pyrosequencing, polymerase chain reaction (PCR)–based methods (easy PGX ready EGFR kit, Diatech Pharmacogenetics, Jesi, Italy; cobas EGFR Mutation Test v2, Roche, Basel, Switzerland; EGFR mutation analysis kit EntroGen, EntroGen, Woodland Hills, CA; Scorpion-ARMS EGFR Plasma RGQ PCR Kit, Qiagen, Hilden, Germany), mass spectrometry-based methods (Sequenom MassARRAY, Diatech Pharmacogenetics, Jesi, Italy), or next generation sequencing.

Techniques: Mutagenesis

Journal: The Oncologist

Article Title: First-Line Osimertinib in Patients with EGFR -Mutant Advanced Non-Small Cell Lung Cancer: Outcome and Safety in the Real World: FLOWER Study

doi: 10.1002/onco.13951

Figure Lengend Snippet: Diagnostic-therapeutic pathway

Article Snippet: For analyses on tissue sample, tumor DNA was extracted from formalin-fixed paraffin-embedded (FFPE) tumor slices through QIAamp DNA FFPE kit (Qiagen, Hilden, Germany); DNA sequencing was carried out with Sanger sequencing, pyrosequencing, polymerase chain reaction (PCR)–based methods (easy PGX ready EGFR kit, Diatech Pharmacogenetics, Jesi, Italy; cobas EGFR Mutation Test v2, Roche, Basel, Switzerland; EGFR mutation analysis kit EntroGen, EntroGen, Woodland Hills, CA; Scorpion-ARMS EGFR Plasma RGQ PCR Kit, Qiagen, Hilden, Germany), mass spectrometry-based methods (Sequenom MassARRAY, Diatech Pharmacogenetics, Jesi, Italy), or next generation sequencing.

Techniques: Diagnostic Assay, Mutagenesis

Journal: The Oncologist

Article Title: First-Line Osimertinib in Patients with EGFR -Mutant Advanced Non-Small Cell Lung Cancer: Outcome and Safety in the Real World: FLOWER Study

doi: 10.1002/onco.13951

Figure Lengend Snippet: Baseline clinical features of patients enrolled

Article Snippet: For analyses on tissue sample, tumor DNA was extracted from formalin-fixed paraffin-embedded (FFPE) tumor slices through QIAamp DNA FFPE kit (Qiagen, Hilden, Germany); DNA sequencing was carried out with Sanger sequencing, pyrosequencing, polymerase chain reaction (PCR)–based methods (easy PGX ready EGFR kit, Diatech Pharmacogenetics, Jesi, Italy; cobas EGFR Mutation Test v2, Roche, Basel, Switzerland; EGFR mutation analysis kit EntroGen, EntroGen, Woodland Hills, CA; Scorpion-ARMS EGFR Plasma RGQ PCR Kit, Qiagen, Hilden, Germany), mass spectrometry-based methods (Sequenom MassARRAY, Diatech Pharmacogenetics, Jesi, Italy), or next generation sequencing.

Techniques: Mutagenesis